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Biophysics

All living cells in order to survive and to perform their physiological functions continuously exchange various atoms and molecules with the extracellular medium. Of particular importance are ions such as sodium, potassium, or calcium. Their controlled exchange with the extracellular medium is crucial to action potentials in neurons, muscle contraction, etc. Since the cellular membrane is normally impermeable to ions, their exchange is facilitated by special proteins called the ion channels, which are embedded in the membrane and form gated microscopic pores.

The focus of our research is to better understand the function of these proteins and their nonequilibrium properties. We know they can detect certain environmental factors, such as changes in electric field, presence of cer

tain ligands or even mechanical stress, and can open or close in response to these factors (ion channel gating). This way they can control and regulate various physiological processes. We use the experimental technique of patch-clamping and recent advances in mathematics and statistical physics to better characterize and control the process of channel gating. We also look at the interaction of inorganic nanoparticles, e.g. multiferroic nanoparticles, with biological cells.

Current Projects:

  • Remote control of voltage-sensing biological macromolecules using multiferroic nanoparticles - with L. Malkinski (University of New Orleans)
  • The effect of membrane cholesterol on gating of ion channels - with J. Shanata (Cornell College) 
  • Conductance hysteresis in ion channels - with A. Walker (University of Central Arkansas)
  • Experimental detection of nonequilibrium kinetic focusing in voltage-gated ion channels
  • Nonequilibrium kinetic focusing and stochastic resonances in Markov models of voltage-gated ion channels - with L. Celardo and F. Borgonovi (University of Brescia, Italy) and L. Kaplan (Tulane University)
  • Optimization of wavelet-based voltage protocols for ion channel electrophysiology

Undergraduate Research:

Biophysics research combines experiments, computations, and theoretical analysis. Student researchers in the Biophysics lab can choose between doing experiments (preparing biological samples, performing patch-clamping experiments) and computational work (analysis of raw experimental data generated from patch-clamping experiments, simulation of ionic currents, and building models of channel gating kinetics). Our experiments use modern ion channel electrophysiology methods, such as the patch clamping technique. The lab is equipped with two patch-clamping stations, one of which is devoted to student training. Most of the numerical simulations are done using MATLAB.

Current student members of the lab:

  • Ariel Hall (Physics'20)
  • Cole Green (Physics'20)
  • Kaough Baggett (Physics'18)

Former lab members include:​​

  • Andrew Eddins (Physics'18)
  • Ilyes Benslimane (Physics'17)
  • Antonio Ayala (Physics'17)
  • Dustin Lindberg (Physics'14)
  • Douglas Alexander (Physics'14)
  • Michael Kammer (Physics'12)
  • David Vumbaco (Physics'12)
  • Warner Sevin (Physics'11)
  • Stella von Meer (Physics'09)
  • Meagan Relle (Biology'08)

Recent publications from the Lab:

  • A. Kargol, L. Malkinski, R. Eskandari, M. Carter, D. Livingston: “Cellular Defibrillation”: Interaction of Microscale Electric Field with Voltage Gated Ion Channels. J. Biol. Phys. (2015)
  • A. Ayala, J.D. Alexander, A.U. Kargol, L. Malkinski, A. Kargol: Piezoelectric micro- and nanoparticles do not affect growth rates of mammalian cells in vitro. J. Bionanosci. 8 (2014) 309-312
  • L. Ponzoni, G.L. Celardo, F. Borgonovi, L. Kaplan, A. Kargol: Focusing in Multiwell Potentials: Applications to Ion Channels. Phys. Rev. E 87 (2013) 852137
  • A. Kargol: Wavelet-based protocols for ion channel electrophysiology. BMC Biophysics 6:3 (2013)
  • A. Kargol, L. Malkinski, G. Caruntu: Biomedical applications of multiferroic particles. In: Advanced Magnetic Materials, InTech (2012)
  • A. Kargol, M. Kargol: Passive transport processes in cellular membranes. In: Porous media: Applications in biological systems and biotechnology, Taylor and Francis Group, LLC (2011)